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2013年07月德宏民族植物学...	2087

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主题	分子生物学数据
标题	马蓝基因组数据服务平台
标题(英)	Strobilanthes Cusia Genome Database
作者	Wei Xu, Libin Zhang, Anthony B. Cunningham, Shan Li, Huifu Zhuang, Yuhua Wang, and Aizhong Liu
作者(英)	Wei Xu, Libin Zhang, Anthony B. Cunningham, Shan Li, Huifu Zhuang, Yuhua Wang, and Aizhong Liu
关键词	genome, indigo, indigo-rich plant, Strobilanthes cusia, transcriptome
关键词(英)	genome, indigo, indigo-rich plant, Strobilanthes cusia, transcriptome
摘要	昆明植物所民族植物学团队研究员王雨华课题组和木本资源发掘与农林复合系统构建团队研究员刘爱忠课题组合作，开展了板蓝的精细基因组测序研究并揭示靛蓝生物合成的分子机制。研究人员结合MinION单分子测序和Hi-C测序技术，首次获得了板蓝染色体水平的精细基因组（图1c，板蓝基因组网站）。利用代谢组分析发现，靛蓝色素：靛苷（indican)、靛蓝（indigo）和靛玉红（indirubin）主要在板蓝的叶片和茎中累积。结合代谢组和转录组的关联分析发现，与靛蓝色素合成途径相关的酶基因，如细胞色素P450、UDP-葡萄糖基转移酶和β-葡萄糖苷酶（图1d），其叶和茎的变化显著。比较基因组学进一步表明了细胞色素P450和UDP-葡萄糖基转移酶基因家族经历了数量上的扩张和功能的分化。该研究揭示了板蓝累积靛蓝的分子基础。
摘要(英)	Natural plant dyes have been developed and used across many traditional societies worldwide. The blue pig-ment indigo has seen widespread usage across South America, Egypt, Europe, India and China for thousandsof years, mainly extracted from indigo-rich plants. The utilization and genetic engineering of indigo in indus-tries and ethnobotanical studies on the effects of cultural selection on plant domestication are limited due tolack of relevant genetic and genomic information of dye plants. Strobilanthes cusia (Acanthaceae) is a typicalindigo-rich plant important to diverse ethnic cultures in many regions of Asia. Here we present a chromo-some-scale genome for S. cusia with a genome size of approximately 865 Mb. About 79% of the sequenceswere identiﬁed as repetitive sequences and 32 148 protein-coding genes were annotated. Metabolic analysisshowed that the main indigoid pigments (indican, indigo and indirubin) were mainly synthesized in the leavesand stems of S. cusia. Transcriptomic analysis revealed that the expression level of genes encoding metabolicenzymes such as monooxygenase, uridine diphosphate-glycosyltransferase and b-glucosidase were signiﬁ-cantly changed in leaves and stems compared with root tissues, implying their participation in indigo biosyn-thesis. We found that several gene families involved in indigo biosynthesis had undergone an expansion innumber, with functional differentiation likely facilitating indigo biosynthesis in S. cusia. This study providesinsight into the physiological and molecular bases of indigo biosynthesis, as well as providing genomic datathat provide the basis for further study of S. cusia cultivation by Asia’s traditional textile producers.
提交者	zhuanghuifu@mail.kib.ac.cn
提交时间	2021/10/25 16:17:08
联系方式	zhuanghuifu@mail.kib.ac.cn

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Xu W , Zhang L , Cunningham A B , et al. Blue genome: chromosome‐scale genome reveals the evolutionary and molecular basis of indigo biosynthesis in Strobilanthes cusia[J]. The Plant Journal, 2020.

Wei Xu, Libin Zhang, Anthony B. Cunningham, Shan Li, Huifu Zhuang, Yuhua Wang, and Aizhong Liu (2021). 马蓝基因组数据服务平台 . Scientific Data Center, Kunming Institute of Botany, CAS. https://data.iflora.cn/Home/DataContent?data_gd=ba08e8a0-64db-0c5f-3350-a2026f244f71