资源信息
| 主题 | 分子生物学数据 |
| 项目类型 | 国家自然科学基金 |
| 项目名称 | 蓖麻转录组和组蛋白修饰数据 |
| 标题 | 蓖麻转录组和表观组数据 |
| 标题(英) | The transcriptome and epigenome data for castor bean |
| 作者 | 徐伟，韩冰，吴迪 |
| 作者(英) | Wei Xu, Bing Han, Di Wu |
| 关键词 | 转录组，表观组，DNA甲基化化，组蛋白修饰 |
| 关键词(英) | transcriptome, epigenome, DNA methylation, Histone modifications, castor bean |
| 摘要 | 我们调查了16个蓖麻组织的基因表达谱；利用WGBS测序了五个组织的DNA甲基化；利用ChIP-seq检测了5个组蛋白修饰在叶片和胚乳的结合位点。 |
| 摘要(英) | We present a gene expression atlas covering 16 representative tissues and identified 1162 seed‑specific genes in castor bean (Ricinus communis), a valuable model for the study of seed biology in dicots. Upon whole‑ genome DNA methylation analyses, we detected 32,567 DMVs across five tissues, covering ~33% of the castor bean genome. These DMVs are highly hypomethylated during development and conserved across plant species. We found that DMVs have the potential to activate transcription, especially that of tissue‑specific genes. Focusing on seed development, we found that many key developmental regulators of seed/endosperm development, including AGL61, AGL62, LEC1, LEC2, ABI3, and WRI1, were located within DMVs. ChIP‑seq for five histone modifications in leaves and seeds clearly showed that the vast majority of histone modification peaks were enriched within DMVs, and their remodeling within DMVs has a critical role in the regulation of seed‑specific gene expression. Importantly, further experiment analysis revealed that distal DMVs may act as cis‑regulatory elements, like enhancers, to activate down‑ stream gene expression. |
| 提交者 | 徐伟 |
| 提交时间 | 2023/5/9 14:08:26 |
| 联系方式 | xuwei@mail.kib.ac.cn |
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